Regulatory

Part:BBa_J100178:Design

Designed by: Rosalind Major   Group: Campbell M Lab   (2014-09-11)


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Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added 1 base pair of G prior to the 3' to 5' sticky end so that the designated number of base pairs between the promoter and transcription initiation site would be met, as 5 were necessary and we only had 4 with the sticky ends.


Source

http://www.karger.com/Article/FullText/354311

References